東京大学理学系研究科の伊藤創祐と申します. クロスポストご容赦ください.
以前通知させて頂いた佐野研セミナー＠東大理のセミナーの場所と時間が変更となります.
場所は小柴ホールに、時間は16:00からとなります.
宜しくお願い致します.
---Sano Lab. Seminar at Hongo, The Univ. of Tokyo---
Speaker: Laurent Blanchoin (Institute of Life Sciences Research and Technologies, CEA/CNRS,
Grenoble, France)
Date: Nov. 1st (Fri.), 16:00-17:30
Place: Science Building 1, Koshiba Hall (理学部1号館、小柴ホール)
Title: Directed Actin Self Assembly, Contractility and Motility
Abstract:
The organization of actin filaments into higher-ordered structures
governs eukaryotic cell shape and movement. Global actin network size
and architecture is maintained in a dynamic steady-state through
regulated assembly and disassembly. We have developed a micropatterning
method that enables the spatial control of actin nucleation sites for in
vitro assays (Reymann, Nat. Mat., 2010). These actin templates were used
to evaluate the response of oriented actin structures to myosin-induced
contractility. We determine that myosins selectively contract and
disassemble anti-parallel actin structures while parallel actin bundles
remain unaffected. In addition, the local distribution of nucleation
sites and the resulting orientation of actin filaments regulate the
scalability of the contraction process. This "orientation selection"
mechanism for selective contraction and disassembly reveals how the
dynamics of the cellular actin cytoskeleton is spatially controlled by
actomyosin contractility (Reymann et al., Science, 2012). Further
application of the micropatterning method will be presented in
particular recent data on the reconstitution of a lamellipodium-type of
actin organization and the fabrication of three-dimensional electrical
connections by means of directed actin self-organization (Galland et
al., Nat. Mat., 2013).
1. Reyman et al., Nucleation geometry governs ordered actin networks
structures, Nature Materials 9, 827–832 (2010).
2. Reyman et al., Actin Network Architecture Can Determine Myosin Motor
Activity, Science 336, 1310-1314 (2012).
3. Galland et al., Fabrication of three-dimensional electrical
connections by means of directed actin self-organization, Nature
Materials 12, 416–421 (2013).